(To the tune of “Downtown”) / Recording by Barbara and Neal Gladstone
Reactions alone
Could starve your cells to the bone
Thank God we all produce
Enzymes!
Units arrange
To make the chemicals change
Because you always use
Enzymes!
Sometimes mechanisms run like they are at the races
Witness the Kcat of the carbonic anhydrases
How do they work?
Inside of the active site
It just grabs onto a substrate
and squeezes it tight
In an
ENZYME!
CAT-al-y-sis
In an
ENZYME!
V versus S
In an ENZYME!
All of this working for you
Energy peaks
Are what an enzyme defeats
In its catalysis
Enzymes!
Transition state
Is what an enzyme does great
And you should all know this
Enzymes!
Catalytic action won’t run wild – don’t get hysteric
Cells can throttle pathways with an enzyme allosteric
You know it’s true
So when an effector fits
It will just rearrange
all the sub-u-nits
Inside an ENZYME!
Flipping from R to T
ENZYME!
Slow catalytically
ENZYME!
No change in Delta G
You should relax
When seeking out the Vmax though
There are many steps
Enzymes!
Lineweaver Burk
Can save a scientist work
With just two intercepts
Enzymes!
Plotting all the data from kinetic exploration
Let’s you match a line into a best fitting equation
Here’s what you do
Both axes are inverted then
You can determine Vmax and
Establish Km
for your
ENZYMES!
Sterically holding tight
ENZYMES!
Substrates positioned right
ENZYMES!
Inside the active site
Enzymes
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